Start by checking how long you're letting the conditioner sit in the new water before it goes in the tank. Some folks don't realize that water conditioners need a few minutes to actually work - if you're pouring treated water straight in, the chemical might not have bonded with chlorine/chloramine yet, especially if your water utility switched to chloramine recently. That's a sneaky one because it seems like nothing changed on your end.
Beyond temperature matching (which the others covered), there's something practical worth trying: do a smaller water change next time, like 15-20% instead of whatever you normally do, and space it out over an hour by adding the water slowly in stages. This gives the fish time to acclimate gradually instead of getting shocked, and it also gives you a chance to see exactly when they start acting stressed. If they're fine with a slow small change but freak out with your usual amount, you've narrowed down whether it's the volume, the speed, or something actually wrong with the water itself.
One more thing - if you've recently switched where you're storing your water or started using a new bucket or container, that could be an issue. Old plastic buckets can leach stuff, or if you're using something that had chemicals in it before, that'll cause problems fast. Keep your water change supplies separate and clearly labeled.